Journal: International Journal of Medical Sciences

Article Title: Cedrus atlantica Extract Suppress Glioblastoma Growth through Promotion of Genotoxicity and Apoptosis: In Vitro and In Vivo Studies

doi: 10.7150/ijms.54468

Figure Lengend Snippet: Primary antibodies information.

Article Snippet: p-Chk2 , Biorbyt Ltd. , Orb5862 , Rabbit , WB: 1/1000.

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Hepatitis B virus X stimulates redox signaling through activation of ataxia telangiectasia mutated kinase

doi:

Figure Lengend Snippet: Phosphorylation of ATM is oxidant-dependent. (A and B) Cells were treated with H2O2 (100 μM) for 30 min or with antioxidant NAC (5 mM) for 2 h. Levels of phospho-ATM (p-ATM) and phospho-Chk2 (p-Chk2) were determined by western blotting. Columns represent relative band intensities normalized against β-actin expressed as fold changes relative to control plasmid-transfected cells (a*, p<0.05 vs. mock plasmid transfected cells; b*, p<0.05 vs. pHBX-transfected cells). (C) Immunohistochemical staining of p-ATM and p-Chk2 in livers. Arrows indicate p-ATM- or p-Chk2-positive non-parenchymal cells (magnification x 40). Columns show labeling indices in hepatocytes. Data are expressed as mean ± SD of three independent experiments (a*, p<0.05 vs. wild mice; b*, p<0.05 vs. HBX transgenic mice).

Article Snippet: Tissue sections were reacted with antibodies against phospho-ATM (Ser1981) (Abgent, San Diego, CA, USA; 1:100) or phospho-Chk2 (Thr68) (Abnova, Taipei, Taiwan) overnight at 4°C using a Vector Elite ABC kit (Vector Laboratories, Burlingame, CA, USA).

Techniques: Western Blot, Control, Plasmid Preparation, Transfection, Immunohistochemical staining, Staining, Labeling, Transgenic Assay